Genes with Cold Shock Domain from Eutrema salsugineum (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (Brassica napus L.) Plants

The increased demand in vegetable oil for food purposes and high-protein feed livestock poultry encourages producers to expand the production of various crops, while occupying rather cold agroclimatic zones. Improved frost resistance cultivated crops would significantly increase yield range rape cultivation a number climate regions. Nine transgenic lines winter containing genes encoding proteins with shock domain (CspA и EsCSDP3) were obtained as result Agrobacterium transformation. In total, 260 explants involved transformation using pBI121-CSPA-plant, efficiency 2.3%; among 750 pBI-EsCSDP3 construction, was 0.4%. As studies, it shown that expression new gene Escsdp3 from plant Eutrema salsugineum able plants effectively cspa E. coli, which is classically used this purpose. analysis T1 generation revealed four resistant (three cspA-plant one line gene). transfer into led significant plants. Two rapeseed freezing (with Non-hardened remained viable after 24 h exposure negative temperatures up −5 °C, passed through hardening stage survived at −16 °C.